A perspective on partially hydrolyzed protein infant formula in nonexclusively breastfed infants / 소아과

The World Health Organization recommends that infants should be exclusively breastfed for the first 6 months of life to provide optimal nutrition in this critical period of life. After this, infants should receive nutritionally adequate and safe complementary foods while breastfeeding continues for up to 2 years of age or beyond. For nonbreastfed infants, infant formula is an available option to provide the nutrition needed. Infant formula is usually prepared from industrially modified cow's milk and processed to adjust for the nutritional needs of infants. However, cow's milk is one of the most common causes of food allergy, affecting 2%–5% of all formula-fed infants during their first year of life. One strategy to prevent cow's milk allergy in nonbreastfed infants is the use of partially hydrolyzed formula (pHF) in high-risk infants, which are infants born in families with atopic disease. However, based on an epidemiological study, approximately half of the infants who develop allergy are not part of the at-risk group. This is because the non-at-risk group is significantly larger than the at-risk group and the non-at-risk infants have approximately 15% risk of developing allergies. This study aimed to evaluate the effects of partially hydrolyzed whey formula (pHF-W) in nonbreastfed infants and determine whether pHF-W can prevent atopic disease in high-risk infants and can be used as routine starter formula regardless of the allergy risk status.

Innovative Dietary Intervention Answers to Baby Colic / 대한소아소화기영양학회지

PURPOSE: The purpose of this paper is to evaluate the efficacy of a lactose- reduced synbiotic partial whey hydrolysate in formula fed infants presenting with colic and the impact of this dietary intervention in mean crying time and quality of life. METHODS: Forty infants with infantile colic were treated during one month with parental reassurance and the intervention formula (partial whey hydrolysate, reduced lactose, Bifidobacterium lactis BB12 and galacto-oligosaccharides) and were compared to a control group of 20 infants with infantile colic treated with parental reassurance and a standard infant formula. Parents completed a quality of life (QoL) questionnaire assessing the burden of infantile colic. Wilcoxon test, t-test and Mann-Whitney test were used to compare QoL scores before and after intervention as well as between the intervention and control group. RESULTS: At inclusion, duration of crying did not differ between both groups. Crying duration decreased with 2.7 hours (from 3.2 to 0.5 hours) in the intervention group while duration of crying decreased only with 1.2 hours in the control group (p<0.001). Stool composition became looser in the intervention group, but defecation frequency did not change. The median scores of the QoL questionnaire improved significantly in the intervention group for all parameters. In the control group, parameters improved significantly also but not for the parent-child and social interaction. The score changes were significantly greater in the intervention than in the control group. CONCLUSION: The intervention formula (partial whey hydrolysate, synbiotic, reduced lactose) significantly reduced the duration of crying and improved QoL of the parents and infants.

Comparison of Antigenicities of Whole Milk and Partial Hydrolysate of Cow's Milk Proteins by Western Blot Analysis / 소아알레르기및호흡기학회지

For the prevention or the management of milk allergy in infancy, partial or extensive hydrolysates of cow's milk have been used in western countries for a couple of decades. Recently a Korean product of partial hydrolysate(HA-21) became available for the prevention of sensitization to cow's milk proteins. In this study, to compare the antigenicities of whole milk(WM) and HA-21, we performed the IgG and IgE western-blot analysis. Sera were obtained from 17 milk sensitive infants and 2 controls, and crude extract of WM and HA-21 and purified beta-lactoglobulin(BLG), bovine serum IgG(B-IgG), alpha-lactalbumin(ALA) were used for blot- inhibition study. After the non-reduced SDS-PAGE, western-blot studies were done using biotinylated anti-human IgG and IgE antibodies, and the reaction were detected by avidine-phosphatase system. By the SDS-PAGE analysis, WM were separated into bovine-IgG(B-IgG), bovine serum albumin(BSA), 56 KD protein, caseins(CAS), BLG and ALA, but there was no visible bands above 14 KD in the case of HA-21. In preliminary blot analysis with 4 milk sensitive sera, we found the broad and strong IgE-binding protein band in the range of 150-200 KD in all cases. This fraction, named as 'P-band' in this study, was completely inhibited by BLG and partially inhibited by B-IgG using blot-inhibition study. Using the western blot analysis of WM, the P-band revealed the most prevalent IgG and IgE binding protein in 16 of 17 tested sera. More than 15 bands were identified by IgG-blot of WM, but the IgE biding proteins were P-band(16 sera), BSA(12 sera), 56 KD protein(8 sera), and CAS(5 sera). In the case of HA-21, BSA(8 sera) was the only protein bound with IgE antibody and the reaction was very weak compared to that of WM. In conclusion, the major IgE binding protein appears to be the p-band which might contain the epitopes of B-IgG and BLG in this study. The antigenicity of HA-21 is remarkably reduced compared to WM, but still minute residual antigenicity and allergenicity are remained in the extract of HA-21.